Exonuclease III
Exonuclease III
CAS: 9037-44-9
Molecular Formula:
Exonuclease III - Names and Identifiers
| Name | Exonuclease III |
| Synonyms | EC 3.1.11.2 EXONUCLEASE III Exonuclease III EXONUCLEASE III LE Nuclease, exodeoxyribo-, III |
| CAS | 9037-44-9 |
| EINECS | 232-912-1 |
Exonuclease III - Physico-chemical Properties
| Appearance | buffered aqueous glycerol solution |
| Storage Condition | −20°C |
| Use | This product is for scientific research only and shall not be used for other purposes. |
Exonuclease III - Risk and Safety
| Safety Description | 36 - Wear suitable protective clothing. |
| WGK Germany | 3 |
Exonuclease III - Reference
| Reference Show more | 1. [IF=10.618] Tao Liu et al."In situ fabrication of urchin-like Cu@carbon nanoneedles based aptasensor for ultrasensitive recognition of trace mercury ion."Biosens Bioelectron. 2022 Jun;206:114147 2. [IF=7.514] Xiaodong Cao et al."An ultrasensitive biosensor for virulence ompA gene of Cronobacter sakazakii based on boron doped carbon quantum dots-AuNPs nanozyme and exonuclease III-assisted target-recycling strategy."FOOD CHEMISTRY. 2022 Oct;391:133268 |
Exonuclease III - Introduction
Exonuclease III is derived from E.coli and has 3 '-5' exonuclease activity. It acts on double-stranded DNA and gradually cuts off single nucleotides from the 3 'OH end direction. Each time the enzyme binds to the substrate, only a few nucleotides are removed, resulting in progressive deletion in the DNA molecular group. The best substrate for this enzyme is a flat-ended, 5 'protruding end double-stranded DNA molecule, but it can also act on the defect of double-stranded DNA to degrade DNA molecules from 3' and release 5 'single nucleotides. For the 3'protruding end, especially for more than 4nt, almost no cutting, nor can the thiophosphoryl lipid bond be cut. The activity of exonuclease III depends partly on the helix structure and varies according to the sequence (C>A = T>G). In addition, exonuclease III also has depurine/pyrimidine-endonuclease activity, RnaseH activity and 3 'phosphatase activity. Preparation of single-strand bottom template units for dideoxy sequencing using non-directional nested deletion site-directed mutation chain-specific probes. In a 50 μl reaction system, the amount required to catalyze E.coli DNA to produce 1 nmol of acid soluble product in 30 minutes at 37 ℃ is defined as an active unit. 1X Exonuclease III Buffer10 mM Bis-Tris(pH 7.0),10 mM MgCl2,1 mM DTT. Enzyme storage solution 50 mM Tris-HCl (pH 8.0), 50 mM KCl, 1 mM DTT, 50% Glycerol. Thermal inactivation 70°C 20min. Stored at -20°C, it can be stored for 3 years.
Last Update：2022-10-16 17:40:47
Exonuclease III - Reference Information
| Product description | exonuclease T(Exo T), also known as RNase T, it is a single-stranded RNA or DNA-specific nuclease that requires a free 3 'end of the exonuclease T and removes nucleotides in the 3'-5 'direction. Exonuclease T can be used to generate flat ends from RNA or DNA containing the 3 'protruding ends. This product is a high-purity protein obtained by recombinant expression of Exonuclease T gene, without endonuclease and other Exonuclease contamination. |
| Storage Conditions | at -20°C, can be stored for 3 years. |
| note | exonuclease T has different activities on RNA and DNA. For RNA, 1 unit of exonuclease T can completely digest 1.0 pmol of rA20 as detected by gel electrophoresis under standard reaction conditions. |
Last Update：2024-04-09 21:11:58
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Product Name: Exonuclease III Visit Supplier Webpage Request for quotationTel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
View History
Exonuclease III
5-溴-N,4-二甲基-3-硝基吡啶-2-胺
6-氟-1-甲基-1H-苯并[d]咪唑
129047-05-8
P-DIAZOBENZENESULFONIC ACID
(6-(二甲氨基)吡啶-3-基)硼酸
S-(Trifluoromethyl) 4-methylbenzenesulfonothioate
ETHYL P-TOLYLACETATE
喹唑啉-4-甲醛
3-(甲氧基二甲基甲硅烷基)丙-1-胺
5-溴-N,4-二甲基-3-硝基吡啶-2-胺
6-氟-1-甲基-1H-苯并[d]咪唑
129047-05-8
P-DIAZOBENZENESULFONIC ACID
(6-(二甲氨基)吡啶-3-基)硼酸
S-(Trifluoromethyl) 4-methylbenzenesulfonothioate
ETHYL P-TOLYLACETATE
喹唑啉-4-甲醛
3-(甲氧基二甲基甲硅烷基)丙-1-胺